Regulation of water permeability of collecting ducts in mouse kidney during postnatal development

Autor: Khodus Gr, E. I. Solenov, Ivanova Ln
Rok vydání: 2009
Předmět:
Zdroj: Russian Journal of Developmental Biology. 40:171-175
ISSN: 1608-3326
1062-3604
DOI: 10.1134/s1062360409030060
Popis: Water permeability of epithelial cells and response to vasopressin was studied on isolated fragments of collecting ducts in the kidney of C57BL/6J mice of three age groups (9, 18, and 60–90 days). The coefficient of osmotic water permeability Pf was evaluated from the rate of cell swelling after medium osmolality was changed from 300 to 200 mOsm/l. The Pf value proved to be significantly lower in mice at the age of 9 days than at the age of 18 days, i.e., after the transition to mixed feeding; although Pf at the age of 18 days does not reach the level of adult animals (58.6 ± 7.7, 94.5 ± 8.8, and 168.4 ± 11.8 μM/s, respectively). The antagonist of vasopressin V2 receptors desmopressin at 1 nM significantly increased Pf in both 18-day-old and adult mice but induced no changes in 9-day-old animals. The inhibitor of protein kinase C Ro-31-8220 in the concentration of 100 nM inhibited the desmopressin effect on Pf in 18 day-old and adult mice but did not inhibit the effect of the analog of the vasopressin secondary messenger cAMP, N6,O2-dibutyryl cyclic monophosphate, on Pf of the plasma membrane in collecting duct cells. Thus, the response of collecting duct cells to vasopressin appears at the end of wining and correlates with the increase in unstimulated osmotic water permeability of the plasma membrane in collecting duct cells. The vasopressin signal transduction via V2 receptors is proposed to require the activity of protein kinase C and calcium-dependent systems of intercellular mediators apart from the cAMP-mediated mechanism.
Databáze: OpenAIRE
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