Autor:	A. M. Chulkin, Yu. P. Vinetskii, E. A. Vavilova, V. A. Serebryanyi
Rok vydání:	2002
Předmět:	Signal peptide chemistry.chemical_classification Catabolite repression Biology Applied Microbiology and Biotechnology Biochemistry Molecular biology Amino acid Penicillium canescens chemistry Hydrolase Xylanase Coding region Gene
Zdroj:	Applied Biochemistry and Microbiology. 38:420-426
ISSN:	0003-6838
DOI:	10.1023/a:1019908232700
Popis:	The complete gene xylA that encodes endo-1,4-β-xylanase secreted byPenicillium canescens was cloned and sequenced. The coding region of the gene is separated by eight introns. The protein comprises 302 amino acids of the mature protein and 25 amino acids of the signal peptide. The xylanase of P. canescens belongs to the glycosyl hydrolase family 10. Nucleotide sequences for binding catabolite repression protein CREA and transactivator protein were detected in the promoter region. A set of multicopy strains displaying a seven to eightfold increase in xylanase yield was obtained. The fraction of xylanase in most productive strains amounted to 30–50% of the total secreted protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5edc56035517d2cfc6e6dc22efa5043b https://doi.org/10.1023/a:1019908232700 Zobrazit plný text záznamu Full text from SpringerLink