The major leaf ferredoxin Fd2 regulates plant innate immunity in Arabidopsis
| Autor: | David Mackey, Zhongmin Wei, Guo-Liang Wang, Jinshan Ella Lin, Joshua J. Blakeslee, Haojie Yan, Dingzhong Tang, Kai Zhang, Lu Rui, Hua Shi, Mo Wang, Wanying Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Innate immune system biology Stromule Jasmonic acid fungi food and beverages Soil Science Plant Immunity Plant Science biology.organism_classification 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry Immunity Arabidopsis Pseudomonas syringae Agronomy and Crop Science Molecular Biology Ferredoxin 010606 plant biology & botany |
| Zdroj: | Molecular Plant Pathology. 19:1377-1390 |
| ISSN: | 1464-6722 |
| DOI: | 10.1111/mpp.12621 |
| Popis: | Ferredoxins, the major distributors for electrons to various acceptor systems in plastids, contribute to redox regulation and antioxidant defence in plants. However, their function in plant immunity is not fully understood. In this study, we show that the expression of the major leaf ferredoxin gene Fd2 is suppressed by Pseudomonas syringae pv. tomato (Pst) DC3000 infection, and that knockout of Fd2 (Fd2-KO) in Arabidopsis increases the plant's susceptibility to both Pst DC3000 and Golovinomyces cichoracearum. On Pst DC3000 infection, the Fd2-KO mutant accumulates increased levels of jasmonic acid and displays compromised salicylic acid-related immune responses. Fd2-KO also shows defects in the accumulation of reactive oxygen species induced by pathogen-associated molecular pattern-triggered immunity. However, Fd2-KO shows enhanced R-protein-mediated resistance to Pst DC3000/AvrRpt2 infection, suggesting that Fd2 plays a negative role in effector-triggered immunity. Furthermore, Fd2 interacts with FIBRILLIN4 (FIB4), a harpin-binding protein localized in chloroplasts. Interestingly, Fd2, but not FIB4, localizes to stromules that extend from chloroplasts. Taken together, our results demonstrate that Fd2 plays an important role in plant immunity. |
| Databáze: | OpenAIRE |
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