Development and characterization of ten microsatellite markers for population studies of the native Brazilian oyster Crassostrea gasar

Autor:	Mauro André Damasceno de Melo, Claudia Helena Tagliaro, Adam Rick Bessa da Silva, E. S. Varela, Iracilda Sampaio
Rok vydání:	2012
Předmět:	Oyster education.field_of_study animal structures biology Ecology fungi Population Zoology Mangrove oyster biology.organism_classification Loss of heterozygosity Genetic marker biology.animal Genetics Crassostrea Microsatellite Allele education Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 4:583-586
ISSN:	1877-7260 1877-7252
DOI:	10.1007/s12686-011-9597-y
Popis:	A set of ten polymorphic microsatellite loci were isolated and characterized from a CT/GT enriched genomic library of the Brazilian native oyster Crassostrea gasar (Adanson, 1757). These markers were polymorphic and successfully amplified in 21 individuals from Sao Joao de Pirabas, Para state, Brazil. The number of alleles per polymorphic locus varied from 6 to 18 and values of observed and expected heterozygosity ranged from 0.3333 to 1.0000 and from 0.6655 to 0.9361, respectively. Only one of the ten microsatellite loci exhibited deviation from Hardy–Weinberg equilibrium and no evidence of linkage was detected between loci. These informative genetic markers will be very useful in population genetic studies of the mangrove oyster C. gasar. The ten markers also showed cross-species transferability with the native oyster Crassostrea rhizophorae.
Databáze:	OpenAIRE
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