Safety profile, antimicrobial and antibiofilm activities of a nanostructured lipid carrier containing oil and butter from Astrocaryum vulgare: in vitro studies
| Autor: | Larissa da Silva Silveira, Bruna Klein, Michele Rorato Sagrillo, Roberto Christ Vianna Santos, Thobias Toniolo de Souza, Ana Paula Becker, Roger Wagner, Diego Bezerra de Souza, Pâmella Scharamm Oliveira, Matheus D. Baldissera, Aline Rossato, Walter Paixão de Souza Filho |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | International Journal for Innovation Education and Research. 9:478-497 |
| ISSN: | 2411-2933 2411-3123 |
| DOI: | 10.31686/ijier.vol9.iss5.3113 |
| Popis: | Ethnopharmacological relevance: Tucumã (Astrocaryum vulgare)is a fruit native to the Amazon region. Extracts from the peel and pulp are thought of as promising treatments for bacterial infections. The primary constituents of Tucumã oil and butter possess unsaturated carbon chains that are susceptible to oxidation by light or heat. The oils have high volatility and low aqueous solubility that limits their use without a vehicle. Nanotechnology refers to techniques to solve these problems. Nanostructured lipid carriers (NLC), for example, protect fixed oils degradation by heat or light, as well as from oxidation and evaporation, ensuring greater stability and function, thereby prolonging the useful life of the final product. Study objectives: The objective of this study was to evaluate the hemolytic, cytotoxic, antimicrobial and antibiofilm properties of an NLC containing Tucumã butter and oil soasto improve the solubility and photosensitivity of the compounds, generating better pharmacological efficacy. Materials and methods: The NLC was assessed for stability for 60 days. The cytotoxicity of nanoparticles in peripheral blood mononucleated cells was determined in culture using assays for cell viability, DNA damage, oxidative metabolism and damage to human erythrocytes. Antimicrobial activity was determined using the broth microdilution technique and antibiofilm activity according to standardized protocols. Results: The Tucumã NLC remained stable throughout the evaluated period, with pH between 5.22–5.35, monodisperse distribution (PDI |
| Databáze: | OpenAIRE |
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