Prospectively Isolated Tetraspanin+ Neoblasts Are Adult Pluripotent Stem Cells Underlying Planaria Regeneration
| Autor: | Sean A McKinney, Hua Li, Eric D. Ross, Anoja Perera, An Zeng, Jungeun Park, Kate Hall, Xin Gao, Yongfu Wang, Andrew C. Box, Kai Lei, Alejandro Sánchez Alvarado, Li-Chun Cheng, Erin L. Davies, Wei Wang, Allison Peak, Longhua Guo, Zulin Yu, Craig L. Semerad |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Regeneration (biology) biology.organism_classification General Biochemistry Genetics and Molecular Biology Planaria Cell biology Transplantation 03 medical and health sciences 030104 developmental biology Tetraspanin Schmidtea mediterranea Stem cell Induced pluripotent stem cell Tissue homeostasis |
| Zdroj: | Cell. 173:1593-1608.e20 |
| ISSN: | 0092-8674 |
| Popis: | Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single-cell transplantation to address this long-standing issue. Large-scale scRNA-seq of sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein and marked by a conserved cell-surface protein-coding gene, tetraspanin 1 (tspan-1). tspan-1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody, rescued the viability of lethally irradiated animals after single-cell transplantation. The first prospective isolation of an adult PSC bridges a conceptual dichotomy between functionally and molecularly defined neoblasts, shedding light on mechanisms governing in vivo pluripotency and a source of regeneration in animals. VIDEO ABSTRACT. |
| Databáze: | OpenAIRE |
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