Development of a dual promoter-mediated gene silencing system in Flammulina velutipes
| Autor: | Tian-Jun Zhang, Liang Shi, Ang Ren, Chao Xu, Ai-Liang Jiang, Mingwen Zhao, Hanshou Yu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Endogeny Biology biology.organism_classification Molecular biology Enzyme assay 03 medical and health sciences Transformation (genetics) 030104 developmental biology Dual promoter RNA interference biology.protein Gene silencing Gene Ecology Evolution Behavior and Systematics Flammulina |
| Zdroj: | Mycoscience. 58:181-187 |
| ISSN: | 1340-3540 |
| Popis: | This study aimed to establish an RNAi system based on a dual promoter construct to interfere with the expression of target genes in Flammulina velutipes . In this study, the endogenous laccase gene, which was cloned as a silencing reporter, was introduced into F. velutipes through electroporation-mediated transformation. Our data unequivocally indicate that the dual promoter silencing system significantly reduced the expression and activity of lac6 . Additionally, reductions in lac6 mRNA levels and enzyme activity were correlated (minimum of a 50% reduction). The molecular tools developed in this study should facilitate the functional characterization of genes in this important species. |
| Databáze: | OpenAIRE |
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