Neither age nor osteoarthritis is associated with synovial fluid antioxidant disturbance or depletion in the horse
| Autor: | N C Smith, Christopher M. Deaton, R C Murray, David Marlin, William Henley |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Antioxidant Physiology Thiobarbituric acid Veterinary (miscellaneous) Endocrinology Diabetes and Metabolism medicine.medical_treatment Biophysics Glutathione Biology Ascorbic acid Biochemistry chemistry.chemical_compound Endocrinology chemistry Physiology (medical) Internal medicine Blood plasma medicine TBARS Uric acid Synovial fluid Orthopedics and Sports Medicine |
| Zdroj: | Comparative Exercise Physiology. 6:121-128 |
| ISSN: | 1755-2559 1755-2540 |
| DOI: | 10.1017/s175525400999016x |
| Popis: | Studies investigating the role of oxidative stress in both the ageing process and osteoarthritis (OA) in human beings are limited by the unavailability of samples from healthy subjects. OA occurs naturally in the horse and has been used as a model of human OA. The objective of this study was to determine the effect of ageing and OA on the non-enzymatic synovial fluid antioxidant status of the horse. The concentrations of ascorbic acid, dehydroascorbate (DHA, oxidized ascorbic acid), uric acid, glutathione, α-tocopherol and thiobarbituric acid reactive substances (TBARS) were determined in paired synovial fluid and plasma samples from 25 horses aged between 3 and 25 years. Osteoarthritic lesions were scored from 0 (healthy) to 4 (severe OA). Glutathione was not detectable in synovial fluid. Neither plasma nor synovial fluid antioxidant concentrations were affected by age. Ascorbic acid concentrations in plasma correlated strongly with those in synovial fluid from both healthy ( P P = 0.003). Synovial fluid concentrations of ascorbic acid and uric acid were not influenced by OA compared with healthy joints. However, the concentration of DHA was slightly, but significantly, elevated in synovial fluid from joints with severe OA (95% CI: [2.2, 11.8] μmol l − 1 ; P |
| Databáze: | OpenAIRE |
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