Autor: Maximilian Callaghan, Xiongying Cheng, Ravinder Sardana, Mitra Panahi, Jenny Phipps, Zaman Alli, Illimar Altosaar
Rok vydání: 2003
Předmět:
Zdroj: Molecular Breeding. 12:21-31
ISSN: 1380-3743
Popis: The potential of plant expression systems to produce functional recombinant proteins was used to produce human prohormone insulin-like growth factor-1B (pro-IGF-1B). Insulin-like growth factor-1 (IGF-1) plays a role in normal growth, development and cell division. The analysis of IGF-1 cDNAs predicted two prohormone precursors (pro-IGF-1A and pro-IGF-1B) with distinct C-terminal E domains. The functions of these precursors, and the E-peptides generated on cleavage to mature IGF-1, are unknown. We expressed human pro-IGF-1B in transgenic tobacco plants and to our knowledge this is the first report of the plant-based recombinant prohormone. The plant-expressed pro-IGF-1B caused proliferation and differentiation of human neuroblastoma cell line SH-SY5Y comparable to human IGF-1. This implies a distinct biological role for pro-IGF-1B. It also suggests that pro-IGF-1B may play a role in tumorigenesis. These results are important in view of obtaining a better knowledge of the role of pro-IGF-1B in human neuroblastoma cells and its relationship to IGF-1. The data also confirm the feasibility of using plant expression system as a cheap and safe bioreactor to produce the recombinant protein for further analysis.
Databáze: OpenAIRE
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