Synthesis of glycopolymers with specificity for bacterial strains via bacteria-guided polymerization

Autor:	David M. Haddleton, Gaojian Chen, Ahmed M. Eissa, Ruyan Feng, Yan Luo, John L. Brash, Yan Gu, Hong Chen
Rok vydání:	2019
Předmět:	Strain specificity Biochemistry Polymerization biology 010405 organic chemistry Chemistry High selectivity General Chemistry 010402 general chemistry biology.organism_classification 01 natural sciences Bacteria 0104 chemical sciences
Zdroj:	Chemical Science. 10:5251-5257
ISSN:	2041-6539 2041-6520
DOI:	10.1039/c8sc05561k
Popis:	Identifying probiotics and pathogens is of great interest to the health of the human body. It is critical to develop microbiota-targeted therapies to have high specificity including strain specificity. In this study, we have utilized E. coli MG1655 bacteria as living templates to synthesize glycopolymers in situ with high selectivity. By this bacteria-sugar monomer-aptation-polymerization (BS-MAP) method, we have obtained glycopolymers from the surface of bacteria which can recognize template bacteria from two strains of E. coli and the specific bacteria-binding ability of glycopolymers was confirmed by both bacterial aggregation experiment and QCM-D measurements. Furthermore, the synthesized glycopolymers have shown a powerful inhibitory ability which can prevent bacteria from harming cells in both anti-infection and co-culture tests.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e9235d6e7bd345d3278548d5d74d749 https://doi.org/10.1039/c8sc05561k Zobrazit plný text záznamu