Evaluation of osteogenic cell differentiation in response to bone morphogenetic protein or demineralized bone matrix in a critical sized defect model using GFP reporter mice

Autor:	Farhang Alaee, David W. Rowe, Jay R. Lieberman, Alex G. Dukas, Michael Pensak, Seung-Hyun Hong
Rok vydání:	2014
Předmět:	Genetically modified mouse Demineralized bone matrix Chemistry dBm Gfp reporter Anatomy Bone morphogenetic protein Molecular biology Green fluorescent protein medicine.anatomical_structure medicine Orthopedics and Sports Medicine Pericyte Myofibroblast
Zdroj:	Journal of Orthopaedic Research. 32:1120-1128
ISSN:	0736-0266
DOI:	10.1002/jor.22657
Popis:	We evaluated the osteoprogenitor response to rhBMP-2 and DBM in a transgenic mouse critical sized defect. The mice expressed Col3.6GFPtopaz (a pre-osteoblastic marker), Col2.3GFPemerald (an osteoblastic marker) and α-smooth muscle actin (α-SMA-Cherry, a pericyte/myofibroblast marker). We assessed defect healing at various time points using radiographs, frozen, and conventional histologic analyses. GFP signal in regions of interest corresponding to the areas of new bone formation was quantified using a novel computer assisted algorithm. All defects treated with rhBMP-2 healed. In contrast, the majority of the defects in the DBM (27/30) and control (28/30) groups did not heal. Quantitation of pre-osteoblasts demonstrated a maximal response (% GFP+ cells/TV) in the Col3.6GFPtopaz mice at day 7 (7.2% ± 6.0, p
Databáze:	OpenAIRE
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