SYMPOSIUM - CARDIAC ION CHANNELS AND THEIR MACROMOLECULAR COMPLEXES: ELECTROPHYSIOLOGY AND BEYOND – ON BEHALF OF THE WORKING GROUP ON CARDIAC CELLULAR ELECTROPHYSIOLOGY
| Autor: | Jordi Heijman, Dobromir Dobrev, Lina Badimon, Shokoufeh Ghezelbash, Niels Voigt, Cristina E. Molina |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
medicine.diagnostic_test Immunoprecipitation business.industry Phosphatase Phospholemman Protein phosphatase 2 CTL Endocrinology Western blot Physiology (medical) Internal medicine cardiovascular system medicine Myocyte Cardiology and Cardiovascular Medicine business Protein kinase C |
| Zdroj: | EP Europace. 17:iii88-iii88 |
| ISSN: | 1532-2092 1099-5129 |
| Popis: | 557 Determinants of altered atrial Na+-Ca2+ exchanger function in patients with chronic atrial fibrillation {#article-title-2} Purpose: Dysfunctional Na+-Ca2+ exchanger type-1 (NCX1) is implicated in atrial fibrillation (AF) triggers/drivers, but the mechanisms of NCX abnormalities in AF patients are unknown and were addressed here. Methods: Transient inward current (Iti) was recorded with perforated patch-clamp technique in atrial myocytes from sinus rhythm (Ctl) and chronic AF (cAF) patients. The NCX1 macromolecular complex proteins were detected in atrial lysates, membrane and cytosolic fractions, or immunoprecipitates (IPs) with Western blot. Results: In cAF myocytes, we detected a higher frequency of Iti current (5.21±2.20 vs. 0.49±0.35 Iti min-1 in Ctl, P |
| Databáze: | OpenAIRE |
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