Stability-Indicating LC Method for Assay of Cholecalciferol
| Autor: | Purnima D. Amin, Pirthi Pal Singh, Namita D. Desai, Satishkumar P. Jain |
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| Rok vydání: | 2008 |
| Předmět: |
Detection limit
Chromatography Chemistry Calibration curve Organic Chemistry Clinical Biochemistry Biochemistry High-performance liquid chromatography Dosage form Analytical Chemistry chemistry.chemical_compound Stability indicating Chemical stability Cholecalciferol Quantitative analysis (chemistry) |
| Zdroj: | Chromatographia. 69:385-388 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1365/s10337-008-0914-x |
| Popis: | This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile–methanol–water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4–10 μg mL−1. The method was accurate and precise with limits of detection and quantitation of 64 and 215 ng, respectively. Mean recovery was 100.71%. The method was used for analysis of cholecalciferol in pharmaceutical formulations in the presence of its degradation products and commonly used excipients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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