MiR-15a and Mir-16-1 Affects the Angiogenesis of Multiple Myeloma by Targeting VEGF-A
| Autor: | Jun Fang, Chunyan Sun, Xiao-Mei She, Lei Chen, Zhangbo Chu, Yu Hu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Oncogene
Angiogenesis Immunology Cell Biology Hematology Plasma cell medicine.disease_cause Biochemistry Vascular endothelial growth factor Endothelial stem cell chemistry.chemical_compound Vascular endothelial growth factor A medicine.anatomical_structure chemistry Cell culture Cancer research medicine Carcinogenesis |
| Zdroj: | Blood. 116:4039-4039 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v116.21.4039.4039 |
| Popis: | Abstract 4039 MicroRNAs (miRNAs) are non-coding small RNAs that negatively modulate protein expression at a post-transcriptional level and are deeply involved in the pathogenesis of a variety of cancers. Two miRNAs, miR-15a and miR-16, which act as putative tumor suppressor by targeting the oncogene BCL2, have been implicated in cell cycle, apoptosis and proliferation. Here we investigated the possible role of miR-15a/miR-16 in the angiogenesis of multiple myeloma (MM). Using stem-loop quantitative reverse transcription-PCR analysis, we showed that miR-15a/miR-16 are significantly underexpressed in primary MM cells as well as MM cell lines (RPMI 8226, ARH-77, OPM-2, KM3, U266 and NIH929). The aberrant expression of miR-15a/miR-16 were detected especially in advanced stage multiple myeloma. The expression of miR-15a and miR-16-1 were remarkably lower in stage III MM patients (n=23), whereas it were significantly higher in healthy individuals (n=18) and stage 2, II MM patients (n=14) (P Disclosures: No relevant conflicts of interest to declare. |
| Databáze: | OpenAIRE |
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