Effects of Carbonyl-cyanide m-chlorophenylhydrazone on Mitochondrial Function of H9C2 Cells

Autor: Vu Thi Thu, Vu Thao Hien, To Thanh Thuy, Pham Thi Bich, Dang Thi Ha Trang, Ngo Thi Hai Yen, Bui Thi Van Khanh
Rok vydání: 2017
Předmět:
Zdroj: VNU Journal of Science: Medical and Pharmaceutical Sciences.
ISSN: 2588-1132
DOI: 10.25073/2588-1132/vnumps.4095
Popis: We examined the effects of carbonyl-cyanide m-chlorophenylhydrazone (CCCP) on mitochondrial function of H9C2 cells. Composition of mitochondrial membrane lipids (cardiolipin) and mitochondrial membrane potential was analyzed by fluorescence intensity change of tetramethl rhodamine ethyl ester (TMRE) and 10-nonyl acridine orange (NAO) using the LSM800 confocal microscope. Our results showed that CCCP strongly and simultaneously affected mitochondrial structure and function of H9C2 cells.
Databáze: OpenAIRE