Isolement de fractions antigéniques membranaires de Toxoplasma gondii par l'intermédiaire de microsphères de méthacrylate

Autor:	A Girault, Laget P, Senet Jm, Mauras G
Rok vydání:	1981
Předmět:	Chromatography biology Chemistry Sonication Toxoplasmas Toxoplasma gondii General Medicine biology.organism_classification Methacrylate Microbiology Membrane Membrane protein Isopycnic parasitic diseases General Earth and Planetary Sciences Ultracentrifuge General Environmental Science
Zdroj:	Annales de l'Institut Pasteur / Immunologie. 132:219-230
ISSN:	0769-2625
DOI:	10.1016/0769-2625(81)90030-1
Popis:	The authors describe a method for isolation of Toxoplasma gondii antigenic membrane components. Technical work is described into four points: 1. 1) specific labelling of membrane proteins was realised by DD125ISA; 2. 2) methacrylate microspheres were bound to unbroken toxoplasma membranes by the mean of an indirect technic with antibody molecules (double sandwich); these microspheres were used to modify the membrane density; 3. 3) toxoplasmas bound to microspheres were broken by sonication; 4. 4) microspheres fixed to membrane components were isolated by isopycnic ultracentrifugation on continuous sucrose gradient.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e5efde1fe4a699b888ab8ab8eb45b43 https://doi.org/10.1016/0769-2625(81)90030-1 Zobrazit plný text záznamu