2089-P: Islet GLUT2 Plasticity Improves Glycemic Control in Insulin-Resistant KK Mice but Not T2DM KKAy Mice
| Autor: | Kathy J. Lepard, Korie L. Sondgeroth |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
geography medicine.medical_specialty geography.geographical_feature_category biology Phloretin Endocrinology Diabetes and Metabolism Insulin medicine.medical_treatment Glucose transporter Islet law.invention chemistry.chemical_compound Cytosol Endocrinology chemistry Confocal microscopy law Internal medicine Internal Medicine medicine biology.protein GLUT2 Secretion |
| Zdroj: | Diabetes. 69 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db20-2089-p |
| Popis: | Beta cells secrete insulin in response to glucose entry by GLUT transporters. GLUT are reduced in beta cells from T2DM rodents. We hypothesized that glucose entry into islets would be reduced in T2DM mice. Islets were isolated from euglycemic mice (C57/Bl6, ICR), insulin-resistant but euglycemic mice (4 and 8 wk KK), prediabetic mice (4 wk KKAy), and T2DM mice (8 wk KKAy). Islets were exposed to the fluorescent glucose analogue 2-NBDG in presence or absence of high glucose or the GLUT inhibitor phloretin. Islet fluorescence was visualized using confocal microscopy and quantified from 3-D images using FIJI Image J. GLUT2 protein was determined in isolated islets by ELISA. Islet GLUT2 location was determined using IF sections with blinded observers assigning a score from 0 (cytosol) to 10 (cell membrane). *p Disclosure K.J. LePard: None. K.L. Sondgeroth: None. Funding Midwestern University (to K.J.L.) |
| Databáze: | OpenAIRE |
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