Molecular characterization of the family of choline transporter-like proteins and their splice variants

Autor:	Martial Ruat, Seana O'Regan, François-Marie Meunier, Elisabeth Traiffort
Rok vydání:	2005
Předmět:	biology Alternative splicing Transfection Biochemistry Oligodendrocyte Myelin basic protein Cell biology Choline transporter Cellular and Molecular Neuroscience Myelin medicine.anatomical_structure medicine biology.protein Neuroglia Choline transport Neuroscience
Zdroj:	Journal of Neurochemistry. 92:1116-1125
ISSN:	1471-4159 0022-3042
DOI:	10.1111/j.1471-4159.2004.02962.x
Popis:	We show here that the choline transporter-like (CTL) family is more extensive than initially described with five genes in humans and complex alternative splicing. In adult rat tissues, CTL2-4 mRNAs are mainly detected in peripheral tissues, while CTL1 is widely expressed throughout the nervous system. During rat post-natal development, CTL1 is expressed in several subpopulations of neurones and in the white matter, where its spatio-temporal distribution profile recalls that of myelin basic protein, an oligodendrocyte marker. We identified two major rat splice variants of CTL1 (CTL1a and CTL1b) differing in their carboxy-terminal tails with both able to increase choline transport after transfection in neuroblastoma cells. In the developing brain, CTL1a is expressed in both neurones and oligodendroglial cells, whereas CTL1b is restricted to oligodendroglial cells. These findings suggest specific roles for CTL1 splice variants in both neuronal and oligodendrocyte physiology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e4a5891a4323481f02b30d6d7b4259a https://doi.org/10.1111/j.1471-4159.2004.02962.x Zobrazit plný text záznamu Plný text