Optimized allylamine deposition for improved pluripotential cell culture
| Autor: | E. Punzón-Quijorna, A. Muñoz Noval, A. Climent Font, D. Gallach Pérez, Raúl Gago, J.P. García Ruiz, Vanessa Sánchez-Vaquero, M. Manso Silván, G. Ceccone |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Plasma activation technology industry and agriculture Analytical chemistry equipment and supplies Condensed Matter Physics Surfaces Coatings and Films Allylamine chemistry.chemical_compound X-ray photoelectron spectroscopy chemistry Chemical engineering Plasma-enhanced chemical vapor deposition Monolayer Polycaprolactone Deposition (phase transition) Wetting Instrumentation |
| Zdroj: | Vacuum. 85:1071-1075 |
| ISSN: | 0042-207X |
| Popis: | Deposition of allylamine (ALL) by plasma enhanced chemical vapor deposition has been optimized on silicon based models. Simultaneous energy recoil detection analysis and Rutherford backscattering spectra show that 100 W deposition is ideal in terms of polymerized film formation and H content while, lower or higher power induce reduced film retention or excessive cross linking, respectively. Surface composition of the ALL film was further probed by X-ray photoelectron spectroscopy revealing a monocomponent N 1s spectrum compatible with the presence of primary amines. Optimized ALL films were applied to polycaprolactone (PCL) surfaces after Ar plasma activation with implications in the chemistry and wettability of this biocompatible polymer. Human mesenchymal stem cells (hMSCs) were cultured on ALL coated PCL surface and controls. ALL functionalized PCL was found to be especially attractive for the formation of confluent monolayers of hMSCs after 72 h of culture. |
| Databáze: | OpenAIRE |
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