Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts
| Autor: | Kok-Song Lai, Swee-Hua-Erin Lim, Ngai-Paing Tan, Dhilia Udie Lamasudin, Teik-Kee Leo, Chun-Wai Mai, Swee-Yee Chin, Shun-Kai Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Gel electrophoresis Reactive oxygen species Antioxidant ved/biology medicine.medical_treatment ved/biology.organism_classification_rank.species HEK 293 cells Pharmaceutical Science Molecular biology Clinacanthus nutans chemistry.chemical_compound Enzyme chemistry Cell culture Drug Discovery medicine Sodium dodecyl sulfate |
| Zdroj: | Pharmacognosy Magazine. 16:431 |
| ISSN: | 0973-1296 |
| DOI: | 10.4103/pm.pm_281_19 |
| Popis: | Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. |
| Databáze: | OpenAIRE |
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