Detection of Highly Variable Genome Fragments in Unmapped Reads of Escherichia coli Genomes

Autor:	Helena Skutkova, Vojtech Barton, Matej Bezdicek, Martina Lengerová, Marketa Nykrynova
Rok vydání:	2020
Předmět:	Variable (computer science) medicine Sequence assembly Identification (biology) Bacterial population Computational biology Biology medicine.disease_cause Escherichia coli Genome Genotyping
Zdroj:	Bioinformatics and Biomedical Engineering ISBN: 9783030453848 IWBBIO
DOI:	10.1007/978-3-030-45385-5_51
Popis:	Whole-genome sequencing becomes a powerful tool in the study of closely related bacterial population as a single nucleotides changes can be detected. However, the postprocessing of obtained data still remains problematic. Reference-based assembly of genomes only allows identification of shared parts of genomes. Here, we show a pipeline for de novo assembly of unmapped reads and locating variable regions in it. Identified regions can be used as new markers for bacterial genotyping.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e215f0d82b1c3369c9ed6a5c4e67055 https://doi.org/10.1007/978-3-030-45385-5_51 Zobrazit plný text záznamu