Development of a Highly Efficient Gene Replacement System for an Industrial Strain of Aspergillus oryzae Used in the Production of Miso, a Japanese Fermented Soybean Paste

Autor:	Nami Ishihara, Akihiro Mori, Shoko Yoshino-Yasuda, Masashi Kato, Noriyuki Kitamoto, Osamu Hasegawa
Rok vydání:	2011
Předmět:	Marketing Genetics biology General Chemical Engineering Gene targeting Locus (genetics) biology.organism_classification Marker gene Genome Industrial and Manufacturing Engineering Aspergillus oryzae Exogenous DNA Homologous recombination Gene Food Science Biotechnology
Zdroj:	Food Science and Technology Research. 17:161-166
ISSN:	1881-3984 1344-6606
DOI:	10.3136/fstr.17.161
Popis:	A homologous gene replacement system was developed for an industrial strain of Aspergillus oryzae KBN630 used in miso production. This system was based on a transformation method involving a bidirectional marker gene, pyrG. To increase the integration of exogenous DNA fragments by homologous recombination, the ku70 gene encoding the Ku70 component of the nonhomologous end-joining machinery was removed from the genome of a pyrG deletion mutant derived from A. oryzae KBN630. The efficiency of homologous integration at the amyR locus was more than 90%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e1fe29753374febcd33fe71c254eab6 https://doi.org/10.3136/fstr.17.161 Zobrazit plný text záznamu