Transportation of Preimplantation Porcine Embryos without Cryopreservation Using a Novel Embryo Carrier

Autor:	Sadao Nakano, Koji Nishida, Yuri Hirayama, Koji Yoshioka, Kiyoshi Akiyama, Akinori Fujiya, Tubasa Hoshi, Chie Suzuki, Hiroyoshi Hoshi, Nobutada Sakagami, Koji Misumi
Rok vydání:	2015
Předmět:	animal structures business.industry Significant difference Embryo Cell Biology Biology Insemination Porcine embryos Cryopreservation Biotechnology Andrology Chemically defined medium medicine.anatomical_structure Reproductive Medicine embryonic structures medicine Blastocyst business
Zdroj:	Journal of Mammalian Ova Research. 32:121-127
ISSN:	1347-5878 1341-7738
DOI:	10.1274/jmor.32.121
Popis:	The objective of this study was to develop a transportation system for fresh porcine embryos in a chemically defined medium without reducing their viability using a novel embryo carrier. When embryos were introduced in 0.25-ml straws with porcine blastocyst medium (PBM) and given a vibration load on a shaker for 20 h to mimic transportation conditions at 38 °C, there was no significant difference in survival rates between the vibration and no-vibration groups. Next, an embryo carrier was developed that can maintain interior conditions of 5% CO2 and 38 °C for the transportation of embryos. The embryos on Day 5 after insemination were divided into three groups. In the first group, the embryos were introduced to straws with PBM and the straws were sealed (sealed group). In the second group, the embryos were transported in a tube with a CO2 pouch (CO2 gas group). They were transported by a door-to-door delivery service. In the last group, the embryos were cultured in an incubator (not-transported gr...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e1ee14c1b851a5dc48025df150d1f85 https://doi.org/10.1274/jmor.32.121 Zobrazit plný text záznamu