Development of a herbal mouthwash containing a mixture of essential oils and plant extracts and in vitro testing of its antimicrobial efficiency against the planktonic and biofilm-enclosed cariogenic bacterium Streptococcus mutans
| Autor: | Katerina Vassilatou, Stavros Dritsas, Efstathios Giaouris, Paraskevi Aggelopoulou, Ioanna Dimou, Sofia Damianaki |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
food.ingredient biology 030106 microbiology Biofilm biochemical phenomena metabolism and nutrition Aquatic Science Cetylpyridinium chloride biology.organism_classification Dental plaque medicine.disease Antimicrobial Applied Microbiology and Biotechnology Streptococcus mutans In vitro 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology food chemistry medicine Food science Holy basil Bacteria Water Science and Technology |
| Zdroj: | Biofouling. 37:397-409 |
| ISSN: | 1029-2454 0892-7014 |
| DOI: | 10.1080/08927014.2021.1924693 |
| Popis: | A herbal mouthwash containing essential oils of holy basil and mountain tea, extracts of St John's wort and European goldenrod (Bucovia™) and cetylpyridinium chloride, was developed and in vitro tested for its efficiency against biofilm formation by Streptococcus mutans, together with its eradicating activity against already preformed (48 h with saccharose) streptococcal biofilm. The minimum inhibitory (MIC) and bactericidal concentrations (MBC) of the final formulation, as well as of its individual components, were initially determined. The results revealed that the mouthwash needed to be applied at two-times its MIC (0.63% v.v-1) to completely inhibit biofilm formation by S. mutans, which was otherwise capable of developing a robust biofilm on the tested surface. Once fully developed, the matrix of the biofilm was found to contain a significant amount of exopolysaccharides protecting the cells, being impossible to eradicate even when exposed to pure mouthwash for 15 min, highlighting the great recalcitrance of biofilm-embedded S. mutans. |
| Databáze: | OpenAIRE |
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