A Novel Method of Covalent Lysozyme Immobilization for the Development of Materials for Medical Applications
| Autor: | N. S. Pokrovsky, I. Yu. Adamova, P. A. Levashov, N.L. Eremeev, D. A. Matolygina, O. A. Dmitrieva, E. D. Ovchinnikova |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification biology 010405 organic chemistry Organic Chemistry biology.organism_classification medicine.disease_cause 01 natural sciences Biochemistry Combinatorial chemistry 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry Covalent bond medicine Agarose Bioorganic chemistry Lysozyme Micrococcus luteus Escherichia coli Bacteria |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 45:101-106 |
| ISSN: | 1608-330X 1068-1620 |
| Popis: | Different methods of covalent lysozyme immobilization have been compared to choose an optimal approach to the development of a material suitable for medical applications in extracorporeal therapy. A novel method for lysozyme immobilization on a polymeric agarose matrix has been proposed, which provides the effective action of lysozyme on bacterial cells and eliminates the leakage of the enzyme from the material. The resultant immobilized lysozyme exhibits bacteriolytic activity toward Gram-positive bacteria Micrococcus luteus and Gram-negative bacteria Escherichia coli. During the immobilization of the enzyme, a broadening of the pH optimum of its activity occurs. The compatibility of immobilized lysozyme with human whole blood has been shown. |
| Databáze: | OpenAIRE |
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