Two‐dimensional electrophoresis of liver proteins: Characterization of a drug‐induced hepatomegaly in rats
| Autor: | Beverly F. Maleeff, Stephen J. Newsholme, N. Leigh Anderson, L. W. Schwartz, Sandra Steiner |
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| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Methionine biology Endoplasmic reticulum Clinical Biochemistry Acute-phase protein Biochemistry Molecular biology Analytical Chemistry Proliferating cell nuclear antigen Amino acid chemistry.chemical_compound chemistry Cytoplasm Protein purification biology.protein Immunostaining |
| Zdroj: | ELECTROPHORESIS. 21:2122-2128 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/1522-2683(20000601)21:11<2122::aid-elps2122>3.0.co;2-# |
| Popis: | Two-dimensional electrophoresis (2-DE) of liver proteins was applied to further characterize an unusual drug-induced increase in hepatocellular rough endoplasmic reticulum (RER) in Sprague-Dawley rats given a substituted pyrimidine derivative. Absolute liver weights of drug-treated rats (9.9 +/- 0.4 g) increased above vehicle-treated controls (7.2 +/- 0.2 g) by 37%. Light microscopy revealed diffuse granular basophilia of the hepatocellular cytoplasm, uncharacteristic of hepatocytes and suggested cells rich in ribosomes, which was confirmed by electron microscopy. Immunostaining for cell proliferation, viz., 5-bromo-2'-deoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA), indicated marked hepatocellular proliferative activity. 2-DE of solubilized liver using an ISO-DALT gel system indicated significant (p |
| Databáze: | OpenAIRE |
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