| Autor: |
Ala'ddin M. Almuaibed, Alan Townshend |
| Rok vydání: |
1988 |
| Předmět: |
|
| Zdroj: |
Analytica Chimica Acta. 214:161-172 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/s0003-2670(00)80438-9 |
| Popis: |
Linear and cyclic systems are described for the determination of ethanol (ca. 0.17–30×10−3 M). In the linear system, the solution passes either through a minicolumn of yeast alcohol dehydrogenase (YADH) immobilized on controlled-pore glass or through minicolumns of the immobilized YADH and of yeast aldehyde dehydrogenase immobilized on cyanogen bromide-activated Sepharose-4B. The NADH formed is monitored either spectrophotometrically or spectrofluorimetrically. In the cyclic system, the solution passes through the same enzyme columns, and the NADH produced is monitored similarly before reconversion to NAD+ in a minicolumn of glutamate dehydrogenase immobilized on cyanogen bromie-activated Sepharose-4B in the presence of α-ketoglutarate and ammonium ions also present in the flow system. the sample throughout for both systems is ca. 40 h−1 and 50 h−1 for spectrophotometric and spectrofluorimetric detection, respectively. An on-line double-injection technique is described as an alternative to the cyclic system for limiting the consumption of NAD+. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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