Comparative study of sub-micrometer polymeric structures: Dot-arrays, linear and crossed gratings generated by UV laser based two-beam interference, as surfaces for SPR and AFM based bio-sensing
| Autor: | Gábor Szekeres, T. Csákó, A. Mathesz, Karoly Osvay, Sz. Veszelka, Botond Penke, Anikó Szalai, Zsolt Bor, A. Kőházi-Kis, Mária A. Deli, Mária Csete, Áron Sipos, A. Schmatulla, Othmar Marti |
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| Rok vydání: | 2007 |
| Předmět: |
Materials science
genetic structures business.industry Scattering Surface plasmon Physics::Optics General Physics and Astronomy Resonance Surfaces and Interfaces General Chemistry Condensed Matter Physics Laser Electromagnetic radiation Surfaces Coatings and Films law.invention Optics Interference (communication) law Microscopy Surface plasmon resonance business |
| Zdroj: | Applied Surface Science. 254:1194-1205 |
| ISSN: | 0169-4332 |
| Popis: | Two-dimensional gratings are generated on poly-carbonate films spin-coated onto thin gold–silver bimetallic layers by two-beam interference method. Sub-micrometer periodic polymer dots and stripes are produced illuminating the poly-carbonate surface by p- and s-polarized beams of a frequency quadrupled Nd:YAG laser, and crossed gratings are generated by rotating the substrates between two sequential treatments. It is shown by pulsed force mode atomic force microscopy that the mean value of the adhesion is enhanced on the dot-arrays and on the crossed gratings. The grating-coupling on the two-dimensional structures results in double peaks on the angle dependent resonance curves of the surface plasmons excited by frequency doubled Nd:YAG laser. The comparison of the resonance curves proves that a surface profile ensuring minimal undirected scattering is required to optimize the grating-coupling, in addition to the minimal modulation amplitude, and to the optimal azimuthal orientation. The secondary minima are the narrowest in presence of linear gratings on multi-layers having optimized composition, and on crossed structures consisting of appropriately oriented polymer stripes. The large coupling efficiency and adhesion result in high detection sensitivity on the crossed gratings. Bio-sensing is realized by monitoring the rotated-crossed grating-coupled surface plasmon resonance curves, and detecting the chemical heterogeneity by tapping-mode atomic force microscopy. The interaction of Amyloid-β peptide, a pathogenetic factor in Alzheimer disease, with therapeutical molecules is demonstrated. |
| Databáze: | OpenAIRE |
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