A rapid latex agglutination test for gender identification in the Atlantic bluefin tuna, Thunnus thynnus (Linnaeus)
| Autor: | Franca Acone, Aldo Corriero, Antonio Camarda, R. Zupa, Elisabetta Micera, Gregorio De Metrio, Angela M. Moramarco, Antonia Zarrilli |
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| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
biology business.industry 010604 marine biology & hydrobiology Zoology Aquatic animal 04 agricultural and veterinary sciences Broodstock Aquatic Science biology.organism_classification 01 natural sciences Latex fixation test Fishery Vitellogenin Aquaculture Agglutination assay 040102 fisheries biology.protein 0401 agriculture forestry and fisheries 14. Life underwater Tuna business Thunnus |
| Zdroj: | Aquaculture Research. 41:1396-1401 |
| ISSN: | 1355-557X |
| Popis: | A rapid, one-step agglutination assay has been developed, based on latex particles sensitized with antibodies against vitellogenin (Vtg), aimed at Atlantic bluefin tuna, Thunnus thynnus (Linnaeus) (ABFT), gender identification. The egg-yolk precursor protein Vtg was used as a gender marker for the assay as it is a female-specific protein synthesized during reproductive maturation. The presence of Vtg in the plasma was revealed in 60-120 s through an agglutination reaction by mixing small volumes of ABFT plasma and an anti-Vtg antibody-latex suspension on a microscope slide. The effectiveness of the present test was restricted to the months of May and June, concomitant with high circulating Vtg levels. Because of its rapidity and ease of performance in the field, the present gender identification assay could be useful for broodstock management in the aquaculture industry as well as in tagging studies on wild populations. |
| Databáze: | OpenAIRE |
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