Inorganic mercury and methylmercury determination in polluted waters by HPLC coupled to cold vapour atomic fluorescence spectroscopy
| Autor: | J. Fermín López-Sánchez, Carmen Ibáñez-Palomino, Angels Sahuquillo |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Chromatography
Reducing agent Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Soil Science chemistry.chemical_element Pollution High-performance liquid chromatography Fluorescence Analytical Chemistry Mercury (element) chemistry.chemical_compound chemistry Environmental Chemistry Methanol Cold vapour atomic fluorescence spectroscopy Spectroscopy Acetonitrile Waste Management and Disposal Water Science and Technology |
| Zdroj: | International Journal of Environmental Analytical Chemistry. 92:909-921 |
| ISSN: | 1029-0397 0306-7319 |
| DOI: | 10.1080/03067319.2010.484887 |
| Popis: | A systematic study of Hg2+ and CH3Hg+ (MeHg+) speciation using hyphenated techniques, was performed for high-performance liquid chromatography coupled to on-line UV irradiation and cold-vapour atomic fluorescence spectroscopy (HPLC-UV-CV-AFS). First, a comparative study of the behaviour of three mobile phase compositions (using tetrabutylammonium bromide (TBAB), L-cysteine and ammonium pyrrolidinedithiocarbamate (APDC)) is presented. The separation and detection system was optimised by considering factors that modify fluorescence signal and the separation such as, the addition of different percentages of an organic modifier (methanol (MeOH) and acetonitrile (ACN)) to the mobile phase, the type of reducing agent used (SnCl2 and NaBH4) and the potential memory effects of the material of which the injection system is made (stainless steel, PEEK). The mobile phase selected for its sensitivity was a mixture 80 : 20 MeOH : 0.0015 mol l–1 APDC and 0.01 mol l–1 NH4CH3COO (pH 5.5). The detection and quantification... |
| Databáze: | OpenAIRE |
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