Isolation of nuclei from frozen human skeletal muscle for single nucleus RNA and chromatin assays v1

Autor:	Dinh H Diep, James R.s. Hocker, Sebastian Preissl
Rok vydání:	2021
DOI:	10.17504/protocols.io.bv9dn926
Popis:	This protocol was adapted for the isolation of single nuclei from frozen skeletal muscle tissues for molecular characterization with the SNARE-seq2 assay. References: (1) Preissl et al (2015). Circulation Research. Doi: 10.1161/CIRCRESAHA.115.306337 (2) Hocker et al (2021). Science Advances. Doi: 10.1126/sciadv.abf1444
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5dea76e34133d5cb7f8aeebfd9ff1e40 https://doi.org/10.17504/protocols.io.bv9dn926 Zobrazit plný text záznamu