Cellulomonas algicola sp. nov., an actinobacterium isolated from a freshwater alga
| Autor: | Takuma Hayashi, Misa Otoguro, Hideki Yamamura, Moriyuki Hamada, Tomohiko Tamura, Masayuki Hayakawa, Kazuyo Matsuyama-Serisawa, Yukihiko Serisawa, Takashi Kohda, Youji Nakagawa, Fujitoshi Yanagida |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Strain (chemistry) Fatty acid General Medicine Diamino acid 16S ribosomal RNA biology.organism_classification 010603 evolutionary biology 01 natural sciences Microbiology Algicola 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Glycolipid chemistry Peptidoglycan Cellulomonas Ecology Evolution Behavior and Systematics |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:2723-2728 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003549 |
| Popis: | An actinomycete strain, TKZ-21T, was isolated from a freshwater alga (Chetophoraceae) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach. Cells were Gram-stain positive, aerobic, non-sporulating, motile, and coccoid or short rod-shaped. The strain grew in the presence of 0–4 % (w/v) NaCl, between pH 6–9.4, and over a temperature range of 15–40 °C, with optimum growth at 30 °C. The peptidoglycan type of strain TKZ-21T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge. The predominant menaquinone was MK-9(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids. The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, and the DNA G+C content was 75.6 mol%. On the basis of 16S rRNA gene sequence analysis, strain TKZ-21T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %). The genome orthoANI value between strain TKZ-21T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively. On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas , with the name Cellulomonas algicola sp. nov. The type strain is TKZ-21T (=NBRC 112905T=TBRC 8129T). |
| Databáze: | OpenAIRE |
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