Immunostick assay for medical diagnosis of rheumatoid arthritis
| Autor: | Jae Chul Pyun, Eun Hang Lee, Min Jung Kang, Hye Jin Choi, Byoung-jin Jeon, Hyuk Wan Ko |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.diagnostic_test
business.industry Biomedical Engineering Autoantibody Bioengineering medicine.disease Applied Microbiology and Biotechnology Molecular biology Antigen Rheumatoid arthritis Immunoassay Medicine Elisa method Medical diagnosis business Kappa Statistical correlation Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 16:1248-1253 |
| ISSN: | 1976-3816 1226-8372 |
| DOI: | 10.1007/s12257-011-0106-7 |
| Popis: | An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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