Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells
| Autor: | Katarzyna Stefańska, Záková J, Pavel Ventruba, Michal Jeseta, Igor Crha, Ievgeniia Kocherova, Paul Mozdziak, Wojciech Pieńkowski, Joanna Perek, Rut Bryl |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Apoptosis related genes endocrine system Pregnancy In vitro fertilisation Individual gene medicine.medical_treatment Cell Biology Biology Oocyte medicine.disease In vitro Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Apoptosis 030220 oncology & carcinogenesis medicine Molecular Biology Gene |
| Zdroj: | Medical Journal of Cell Biology. 8:176-182 |
| ISSN: | 2544-3577 |
| DOI: | 10.2478/acb-2020-0023 |
| Popis: | Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points. Running title: Apoptosis-related genes expression in granulosa cells in vitro |
| Databáze: | OpenAIRE |
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