Peptidomics-based study reveals that GAPEP1, a novel small peptide derived from pathogenesis-related (PR) protein of cotton, enhances fungal disease resistance
Autor: | Jianchang Du, Na Yuan, Baolong Zhang, Chen Dai, Xitie Ling |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Antimicrobial peptides Plant Science Fungus Plant disease resistance Gossypium medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences Genetics medicine Verticillium dahliae Molecular Biology Innate immune system biology fungi food and beverages Pathogenic bacteria biology.organism_classification 030104 developmental biology Verticillium wilt Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
Zdroj: | Molecular Breeding. 39 |
ISSN: | 1572-9788 1380-3743 |
Popis: | Peptides play important roles in many important biological processes. For instance, plant antimicrobial peptides, which are essential components of innate immunity and exert rapid defense response, have a broad activity against pathogenic bacteria, fungi, enveloped viruses, and parasites. Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a destructive vascular disease in plants. In this study, we used mass spectrometry approach to detect and characterize important peptides, especially antimicrobial peptides from roots of Gossypium arboretum and to evaluate their inhibitory activities against phytopathogenic fungi. We have identified a novel small peptide, GAPEP1, which is derived from the N-terminal of Pathogenesis-related protein STH-2 protein and upregulated by V. dahliae inoculation. GAPEP1 is localized in the nucleus and cell membrane. Plants with exogenous treatment or overexpressing GAPEP1 both exhibited enhanced disease resistance as compared with the control group. Our results provide the cotton endogenous peptides library that could potentially be used to develop natural, targeted, and environmentally friendly strategies to enhance the resistance of Gossypium species against biotic attackers. |
Databáze: | OpenAIRE |
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