A Monolithic Dielectrophoresis Chip With Impedimetric Sensing for Assessment of Pathogen Viability
| Autor: | Maria V. Riquelme Breazeal, Masoud Agah, Kruthika Kikkeri, Xiang Ren, Amy Pruden |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
business.industry Mechanical Engineering 010401 analytical chemistry Microfluidics 02 engineering and technology Dielectrophoresis 021001 nanoscience & nanotechnology Chip 01 natural sciences Signal 0104 chemical sciences Microelectrode Microfluidic channel Electrode Optoelectronics Electrical and Electronic Engineering 0210 nano-technology business Electrical impedance |
| Zdroj: | Journal of Microelectromechanical Systems. 27:810-817 |
| ISSN: | 1941-0158 1057-7157 |
| Popis: | Here, we report a rapid and low-cost live/dead assay for bacteria in aqueous samples via a dielectrophoresis (DEP) technique employed in a microfluidic platform. The platform has reusable compartment housing the microelectrodes for DEP activation and bioimpedance sensing. The disposable compartment contains the sample inlet and outlet and the microfluidic channel for sample analysis. The chip has an array of insulating pillars for generating DEP forces on bacteria and a focusing region for enhanced impedance signal. The DEP force is utilized to enrich bacteria and selectively release them by tuning the frequency of the applied electric field. An algorithm is implemented to determine the concentration of live bacteria in the mixture by analyzing the magnitude and duration of the measured impedance. The chip performance was validated by analyzing various ratios of live/dead bacteria and was compared with parallel flow cytometry experiments. [2018-0100] |
| Databáze: | OpenAIRE |
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