Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping
| Autor: | Jan Juřica, Ondřej Peš, Ondřej Zendulka, Jan Máchal, Katarína Kostolanská |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Chromatography General Chemical Engineering Lansoprazole Isopropyl alcohol 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences Biochemistry Industrial and Manufacturing Engineering Orders of magnitude (mass) 0104 chemical sciences Triple quadrupole mass spectrometer chemistry.chemical_compound chemistry Liquid chromatography–mass spectrometry Materials Chemistry medicine Ammonium formate 0210 nano-technology medicine.drug |
| Zdroj: | Chemical Papers. 73:2955-2963 |
| ISSN: | 1336-9075 2585-7290 |
| Popis: | A simple and sensitive liquid chromatography method with absorbance or mass spectrometry detection was developed and validated for the determination of lansoprazole, lansoprazole sulfone, and 5-hydroxylansorpazole in human serum with omeprazole as an internal standard. Analytes were, after an extraction step with a mixture of isopropyl alcohol: dichloromethane (2:8, v/v), separated in a gradient elution (acetonitrile: 20 mM ammonium formate) and detected at 280 and 300 nm or by monitoring selective reactions in a triple quadrupole mass analyzer. The limits of detection were, for all analytes, below 2 ng/mL and 3 pg/mL in the LC UV and LC MS method, respectively. The combined linear dynamic range exceeded 5 orders of magnitude. The method was successfully applied in the assessment of CYP2C19 and CYP3A4 metabolic phenotypes in ST-elevated myocardial infarction patients receiving novel anticoagulant treatment. |
| Databáze: | OpenAIRE |
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