| Autor: |
Rémi N. Charrel, Renaud Vincentelli, Shirley Masse, Fabien Durbesson, Bruno Coutard, Xavier de Lamballerie, Alessandra Falchi, Margot Barthelemy, Eva Lopez, Cécile Baronti |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Since the beginning of the Covid-19 pandemics, variants have emerged. Whereas most of them have no to limited selective advantage, some display increased transmissibility and/or resistance to immune response. To date, most of the mutations involved in the functional adaptation are found in the Receptor Binding Module (RBM), close to the interface with the human receptor ACE2. In this study, we thus developed and validated a fast and simple molecular assay allowing the detection and partial identification of the mutations in the RBM coding sequence. After the amplification of the region of interest, the amplicon is heat-denatured and hybridized with an amplicon of reference. The presence of a mutation in the heteroduplex can be cleaved by a mismatch-specific endonuclease and the cleavage pattern is analysed by capillary electrophoresis. The approach was first validated on viral RNA purified different SARS-CoV-2 variants produced in the lab before being implemented for clinical samples. The results highlighted the performance of the assay for the detection of mutations in the RBM from clinical samples. The procedure can be easily set up for high throughput identification of the presence of mutations and serve as a first-line screening to select the samples for full genome sequencing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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