Hairpin Assembly Amplified Electrochemical Biosensor for Highly Sensitive and Specific Detection of DNA
| Autor: | Changli Zhong, Xiaojuan Ding, Jian Zhang, Feihu Ji, Gang Yang, Ye Zhang, Junhong Yang, Nian Wang |
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| Rok vydání: | 2016 |
| Předmět: |
Streptavidin
Chemistry 010401 analytical chemistry Substrate (chemistry) Nanotechnology 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Highly sensitive chemistry.chemical_compound Complementary sequences Biotin Linear range Electrochemistry Biophysics Biosensor DNA |
| Zdroj: | Electroanalysis. 28:1578-1583 |
| ISSN: | 1040-0397 |
| DOI: | 10.1002/elan.201501178 |
| Popis: | In this report, a simple electrochemical biosensor has been developed for highly sensitive and specific detection of DNA based on hairpin assembly amplification. In the presence of target DNA, the biotin-labelled hairpin H1 is opened by hybridizing with target DNA through complementary sequences. Then the opened hairpin H1 assembles with the hairpin H2 to displace the target DNA, generating H1-H2 complex. The displaced target DNA could trigger the next cycle of hairpins assembly, resulting in the generation of numerous H1-H2 complexes. Subsequently, the H1-H2 complex hybridizes with the capture probe immobilized on the electrode. Finally, the streptavidin alkaline phosphatase (ST-ALP) binds to biotin in the capture probe-H1-H2 complex and catalyzes the substrate α-naphthol (α-NP) to produce electrochemical signal. To make a more fascinating hairpin assembly amplification strategy in signal amplification, mismatched base sequences are designed in hairpin H2 to decrease non-specific binding of the hairpin substrates. The developed biosensor achieves a sensitivity of 20 pM with a linear range from 25 pM to 25 nM, and shows high selectivity toward single-base mismatch. Thus, the proposed electrochemical biosensor might have the potential for early clinical diagnosis and therapy. |
| Databáze: | OpenAIRE |
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