Porous SiO 2 ‐coated ultrasmall selenium particles nanospheres attenuate cerulein‐induce acute pancreatitis in mice by downregulating oxidative stress
| Autor: | Jun Jie Fan, Guo Ying Deng, Lun Gen Lu, Ze Hua Huang, Xing Peng Wang, Qi Xiang Mei, Chun Lan Huang, Yue Zeng, Ying Ying Lu, Yang Fu, Jun-Hui Hu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Reactive oxygen species biology business.industry Gastroenterology Glutathione Malondialdehyde medicine.disease_cause Proinflammatory cytokine Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology chemistry 030220 oncology & carcinogenesis Internal medicine Myeloperoxidase medicine biology.protein 030211 gastroenterology & hepatology business Blood urea nitrogen Oxidative stress |
| Zdroj: | Journal of Digestive Diseases. 22:363-372 |
| ISSN: | 1751-2980 1751-2972 |
| Popis: | OBJECTIVE To investigate the potential therapeutic role of porous SiO2 -coated ultrasmall selenium particles nanospheres (Se@SiO2 nanospheres) pretreatment in acute pancreatitis (AP) and to investigate the related mechanism. METHODS C57BL/6 mice were randomized to the normal control (CON) group, the AP (induced by cerulein injection) (CAE) group, and AP pretreated with Se@SiO2 nanocomposites at 1 and 2 mg/kg (CAE + 1 or 2 mg/kg Se@SiO2 ) groups, respectively. Serum levels of amylase and lipase, inflammatory cytokines (interleukin [IL]-6, IL-1β and tumor necrosis factor [TNF]-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr) were measured, and histopathology was performed to examine the tissue samples of the pancreas, lungs, kidneys and liver. Immunofluorescence assay of reactive oxygen species (ROS), myeloperoxidase (MPO) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling were conducted, and levels of MPO, malondialdehyde, superoxide dismutase and glutathione were evaluated. Finally, Western blot analysis was used to evaluate protein expressions of Nrf2, HO-1, NQO1, TLR4, MyD88 and p-p65 in pancreatic tissue. RESULTS Se@SiO2 nanospheres alleviated pathological damage to the pancreas, and reduced pancreatic enzymes and inflammatory cytokines. Injury to other organs such as the liver, lungs and kidneys was also alleviated, as indicated by decreased ALT, AST, BUN, and Cr levels as well as improved histopathology. Moreover, Se@SiO2 nanospheres reduced oxidative stress, and ultimately inhibited TLR4/ MyD88/p-p65 pathway and increased the protein expressions of NQO1, Nrf2, and HO-1. CONCLUSION Se@SiO2 nanospheres may alleviate AP by relieving oxidative stress and targeting the TLR4/Myd88/p-p65 and NQO1/Nrf2/HO-1 pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|