| Autor: |
R. D. Lumsden, Scott M. Lohrke, Daniel P. Roberts, K. P. Hebbar, P.D Dery, Susan L. F. Meyer, Wei Li |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Crop Protection. 21:129-135 |
| ISSN: |
0261-2194 |
| DOI: |
10.1016/s0261-2194(01)00074-6 |
| Popis: |
The potential biocontrol bacterium Burkholderia ambifaria isolate BC-F significantly suppressed damping-off of cucumber and soybean caused by Pythium ultimum when applied as a seed coating. Cucumber and soybean seed coated with a peat bond formulation of isolate BC-F had significantly greater plant stands than the non-treated pathogen controls in soil-less mix. Soybean seeds treated with isolate BC-F had significantly greater plant fresh weight per pot and plant height per pot than the pathogen control. Isolate BC-F effectively colonized the roots of corn, cucumber, soybean, and sunflower and was detected at ⩾10 5 colony-forming-units per gram fresh weight of root after 4 weeks in two different natural soils. Burkholderia ambifaria BC-F grown on nutrient broth agar or potato dextrose agar released a diffusable metabolite(s) that inhibited hyphal growth of P. ultimum, Rhizoctonia solani, Phytophthora capsici , and Fusarium oxysporum f.sp. lycopersici . Culture filtrates from isolate BC-F grown on nutrient broth contained a metabolite(s) that inhibited egg hatch and mobility of second-stage juveniles of Meloidogyne incognita . These culture filtrates contained chitinase and protease activities. However, the M. incognita . The >8 kDa fraction contained no inhibitory activity, indicating that inhibition of egg hatch and mobility of second-stage juveniles of M. incognita was not directly due to chitinase and protease activities. The demonstrated ability of isolate BC-F to suppress disease caused by animal and fungal pathogens of many crop plants may be due to the ability of isolate BC-F to persist for long periods in association with roots of diverse crop plants in different soils and the production of a metabolite(s) with broad spectrum anti-biotic activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect