MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles
| Autor: | Hua Ai, Rongrong Jin, Ye Xu, Wencheng Zhu, Changqiang Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
medicine.diagnostic_test Magnetic resonance imaging Cell migration 02 engineering and technology Dendritic cell 021001 nanoscience & nanotechnology Tracking (particle physics) Cell labeling 03 medical and health sciences 0302 clinical medicine In vivo 030220 oncology & carcinogenesis medicine Stem cell 0210 nano-technology Superparamagnetic iron oxide Biomedical engineering |
| Zdroj: | Cell Tracking ISBN: 9781071603635 |
| Popis: | Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79-90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells. |
| Databáze: | OpenAIRE |
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