A Method for Leukokinetic Study in the Nonsteady State

Autor:	C. R. Bishop, Helen Ashenbrucker, J. W. Athens, Gerald Rothstein
Rok vydání:	1971
Předmět:	Chromatography Chemistry Immunology Kinetics Granulocytosis Cell Biology Hematology Granulocyte Neutropenia medicine.disease Biochemistry Agglutination (biology) medicine.anatomical_structure Nonsteady state medicine Specific activity Early phase
Zdroj:	Blood. 38:302-311
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v38.3.302.302
Popis:	In these studies, the DF32P leukokinetic technique has been modified so that neutrophil kinetics may be studied in the nonsteady state. In order to achieve this modification, an acid citrate solution was used in preparing leukocytes for radioactive assay. This results in a suspension free of cell agglutination so that a specific activity related only to neutrophils is obtained. This technique has been successfully employed in the study of the early phase of cortisol-induced granulocytosis, allowing measurement of cell flow rate in and out of the circulating granulocyte pool. This method is also useful in the study of subjects with pronounced neutropenia.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5d7ba7ed3ff0dc9af54acf4bfaf7f993 https://doi.org/10.1182/blood.v38.3.302.302 Zobrazit plný text záznamu