Development of a liposome immunosorbent assay for human interferon-y
| Autor: | G.W.K. Hugenholtz, W.P. van Bennekom, Auke Bult, P. H. Van Der Meide, H.A.H. Rongen, H.M. van der Horst |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Liposome
medicine.diagnostic_test Chemistry Fluorescence spectrometry Biochemistry Molecular biology Analytical Chemistry Blood cell medicine.anatomical_structure Interferon Biotinylation Immunoassay medicine Environmental Chemistry Interferon gamma Quantitative analysis (chemistry) Spectroscopy medicine.drug |
| Zdroj: | Analytica Chimica Acta. 287:191-199 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/0003-2670(93)e0592-u |
| Popis: | A novel and sensitive liposome immunosorbent assay (LISA) for IFN- y is described. Biotinylated liposomes with encapsulated carboxyfluorescien comprise a universal label for immunoassays. Stability of the liposomes is proven to be adequate for long-term storage, without loss of encapsulated marker. Sensitivity of the LISA of 6×10 −1 M is comparable to the best colorimetric immunoassays for IFN- y , and the linear detection range (6−2400×10 −12 M) exceeds the colorimetric range. Analysis of biological samples with the LISA showed no interference on the liposomes from potential disturbing factors in either plasma or blood cell culture supernatants. |
| Databáze: | OpenAIRE |
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