Is droplet evaporation crucial in the mechanism of electrospray mass spectrometry?

Autor:	R. T. O'Brien, Roger Guevremont, Shier S. Berman, K. W. M. Siu, J. C. Y. Le Blanc
Rok vydání:	1993
Předmět:	Electrospray Chemistry Electrospray ionization Analytical chemistry Evaporation Extractive electrospray ionization Mass spectrometry Biochemistry Sample preparation in mass spectrometry Desorption Molecular Medicine Instrumentation Spectroscopy Ambient ionization
Zdroj:	Organic Mass Spectrometry. 28:579-584
ISSN:	1096-9888 0030-493X
DOI:	10.1002/oms.1210280519
Popis:	Evaporation of solvent from charged droplets was found not to be a prerequisite to ion desorption in electrospray mass spectrometry. Evidence of evaporation was absent in an examination of the electrospray mass spectral profiles of cytochrome c and myoglobin in 0.2% acetic and propionic acid solutions; the pHs of these two acid solutions are expected to change in opposite directions with evaporation. The results strongly suggest that ions, as observed in electrospray mass spectrometry, are desorbed from solutions that have undergone minimal evaporation, in other words, at the beginning rather than later parts of the electrospray process. It is speculated that ions are desorbed directly from the solution-air interface at the needle tip.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5d679405c68af31a88a8f67cfe742089 https://doi.org/10.1002/oms.1210280519 Zobrazit plný text záznamu Plný text