DNA Fragmentation and Nuclear Degradation during Programmed Cell Death in the Suspensor and Endosperm ofVicia faba
| Autor: | Inger Hakman, Björn Walles, Ulla Wredle |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | International Journal of Plant Sciences. 162:1053-1063 |
| ISSN: | 1537-5315 1058-5893 |
| DOI: | 10.1086/321922 |
| Popis: | In Vicia faba, both the suspensor and the endosperm are short‐lived structures. The aim of this work was to elucidate and compare programmed cell death (PCD) mechanisms in these two ephemeral tissues. To achieve this, we used the TdT‐mediated dUDP fluorescent (FITC) nick end labeling (TUNEL) technique to trace DNA fragmentation and transmission electron microscopy to follow chromosomal and nuclear degradation. The TUNEL experiments demonstrated DNA fragmentation in the endosperm nuclei 13 d after pollination (dap) and in the suspensor at 14 dap. However, the ultrastructural studies did not show any chromosomal degradation in the nuclei of the suspensor or endosperm until 17 dap, indicating that the DNA fragmentation is an initial step in the PCD pathway. We have further documented fundamental differences in the degeneration process of the nuclei of the two tissues. In the suspensor the heterochromatin becomes more condensed during degeneration and disperses to the nuclear periphery as electron‐dense areas... |
| Databáze: | OpenAIRE |
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