Cryopreservation of sperm in annual fish Austrolebias minuano (Cyprinodontiformes; Rivulidae)
| Autor: | Daiana Kaster Garcez, Murilo de Oliveira Fernandes, Izani Bonel Acosta, Antonio Sergio Varela Junior, Lizandra Jaqueline Robe, Carine Dahl Corcini, Stela Mari Meneghello Gheller |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chromatography Cryoprotectant Dimethyl sulfoxide 04 agricultural and veterinary sciences Aquatic Science Biology biology.organism_classification Rivulidae Sperm Cryopreservation 03 medical and health sciences chemistry.chemical_compound chemistry 040102 fisheries Glycerol 0401 agriculture forestry and fisheries Killifish Cyprinodontiformes 030304 developmental biology |
| Zdroj: | Aquaculture Research. 51:147-154 |
| ISSN: | 1365-2109 1355-557X |
| DOI: | 10.1111/are.14356 |
| Popis: | The effects of the cryoprotectants dimethyl sulfoxide, glycerol and methyl glycol at different concentrations on Austrolebias minuano sperm quality parameters were evaluated in this study. The cellular kinetic parameters, determined using flow cytometry, indicated the best results with the samples cryopreserved with 7.5% methyl glycol. Dimethyl sulfoxide concentrations of 7.5% and glycerol concentrations of 10%, 12.5% and 15% demonstrated the least benefit across all evaluated sperm kinetic parameters. When assessed using flow cytometry, a concentration of 7.5% methyl glycol similarly showed better sperm kinetic parameters than 12.5% dimethyl sulfoxide. We conclude that cryoprotectants, especially methyl glycol, are effective for the preservation of sperm quality in A. minuano. However, high sensitivity of spermatozoa against glycerol was observed in these studies; thus, it is not recommended for cryopreservation purposes. |
| Databáze: | OpenAIRE |
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