| Popis: |
DOI : 10.26650/experimed.2018. 473615 Objectives : The hypermethylator phenotype is used as a term for both acute myeloid leukemia (AML) and other tumors. We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1, ER, p15, Ecadherin, RAR β , p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs). Material and Method : We analyzed the methylation profiles using methylation specific PCR and COBRA and the expression of DNMTs (DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25 adult AML samples. Results : The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members of the methylator phenotype. Conclusion : Our study demonstrated that the gene DNMT3A was dramatically upregulated and significantly correlated with the methylator phenotype. |
