Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells

Autor:	Neha Kulkarni-Dwivedi, Pratikshkumar R Patel, Bhupendra V Shravage, Rinku D Umrani, Kishore M Paknikar, Sachin H Jadhav
Rok vydání:	2022
Předmět:	Biomedical Engineering Medicine (miscellaneous) General Materials Science Bioengineering Development
Zdroj:	Nanomedicine. 17:1929-1949
ISSN:	1748-6963 1743-5889
DOI:	10.2217/nnm-2022-0171
Popis:	Background: Studies on the anticancer effects of lanthanum strontium manganese oxide (LSMO) nanoparticles (NPs)-mediated hyperthermia at cellular and molecular levels are scarce. Materials & methods: LSMO NPs conjugated with folic acid (Fol-LSMO NPs) were synthesized, followed by doxorubicin-loading (DoxFol-LSMO NPs), and their effects on breast cancer cells were investigated. Results: Hyperthermia (45°C) and combination treatments exhibited the highest (∼95%) anticancer activity with increased oxidative stress. The involvement of intrinsic mitochondria-mediated apoptotic pathway and induction of autophagy was noted. Cellular and molecular evidence confirmed the crosstalk between apoptosis and autophagy, involving Beclin1, Bcl2 and Caspase-3 genes with free reactive oxygen species presence. Conclusion: The study confirmed hyperthermia and doxorubicin release by Fol-LSMO NPs induces apoptosis and autophagy in breast cancer cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5d33ee61f9844f9fca6b8ce3266573e1 https://doi.org/10.2217/nnm-2022-0171 Zobrazit plný text záznamu