Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite
| Autor: | Bart N. Lambrecht, J. C. Vidal-Quist, Pedro Castañera, Félix Ortego, Pedro Hernández-Crespo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Allergy medicine.disease_cause Acariformes Microbiology 03 medical and health sciences 0302 clinical medicine Allergen immune system diseases medicine Mite Bioassay Ecology Evolution Behavior and Systematics House dust mite General Veterinary biology Acaricide Pyroglyphidae respiratory system biology.organism_classification medicine.disease respiratory tract diseases 030104 developmental biology 030228 respiratory system Insect Science Immunology Parasitology |
| Zdroj: | Medical and Veterinary Entomology. 31:97-101 |
| ISSN: | 0269-283X |
| DOI: | 10.1111/mve.12200 |
| Popis: | The expression of allergen genes in house dust mites is influenced by temperature and relative humidity, but little is known of the impacts of other environmental factors that may alter the repertoire of allergens released by mites in home microhabitats. Bioassays were conducted in concave microscope slides in combination with real-time quantitative polymerase chain reaction (RT-qPCR) to analyse gene expression of 17 allergens of Dermatophagoides pteronyssinus (Acariformes: Pyroglyphidae) exposed to three chemical stressors that can be present in domestic environments. Short-term exposure (5-12 days) to diesel exhaust particles (DEPs) (1 µg/cm2 ), bacterial lipopolysaccharide endotoxin (0.1 µg/cm2 ) and benzyl benzoate (3.2 µg/cm2 ), at concentrations exceeding those expected in homes, had no significant effect on allergen transcription. A significant increase in the transcription of allergens Der p 3, Der p 8 and Der p 21 was observed only after exposing mites to a higher concentration of DEPs (10 µg/cm2 ) over a whole generation. In combination, the present results suggest that the analysed factors have low impact on allergen production. The methodology described here offers a sound and rapid approach to the broad-spectrum study of factors affecting allergen-related mite physiology, and allows the simultaneous screening of different factors in a relatively short period with consideration of the full spectrum of allergen genes. |
| Databáze: | OpenAIRE |
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